Role CKAP5 v zprostředkování interakce mezi aktinovým a mikrotubulárním cytoskeletem

Dujava Ždímalová, Michaela

Dynamic changes in cytoskeletal architecture are essential for many crucial events throughout lives of all cells. One of the examples is neuronal pathfinding, ensured by specialized axonal structures enriched in actin and microtubule cytoskeleton, known as neuronal growth cones. Growth cones act as motile sensors and navigators, as the tight regulation of their actin and microtubule cytoskeleton results in directed axonal outgrowth guided towards the proper targets, which is crucial for processes such as synaptogegnesis or regeneration. Recently, protein CKAP5 (previously described as a microtubule + tip polymerase) has been proposed to facilitate actin-microtubule crosstalk in growth cones, indispensable for their proper functioning. Here, we combine the power of in vitro reconstitution assays with total internal reflection fluorescence microscopy to explore the underlying mechanism of CKAP5 actions. Our findings confirm that CKAP5 also associates with actin filaments and indeed, recruits them to the microtubule lattice. Furthermore, we describe a remarkable behavior of dynamic system containing CKAP5 and both, microtubules and actin filaments, wherein actin bundles are templated along dynamic microtubules by CKAP5. Importantly, upon microtubule depolymerization, the corresponding actin bundle can...

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