Úloha genu FAR5 v horské adaptaci Arabidopsis arenosa

Poštulková, Alžběta

The repeated adaptation presents a unique opportunity to study the mechanisms of evolution in natural replicates. Repeated adaptation of Arabidopsis arenosa, a wild relative of the model organism Arabidopsis thaliana, to the alpine environment was previously studied. Genomic analysis by selection scans revealed a set of candidate alleles. Here, I present a functional follow-up study of a candidate alpine-adaptive allele of FAR5. Specifically, I asked: What are the characteristics of the alpine (A) allele compared to the foothill (F) allele? Is there any phenotypic effect of the two different alleles of FAR5? What are the environmental factors shaping the distribution of the alpine allele of FAR5 among the populations of A. arenosa? I first demonstrated that natural variation in FAR5 played a role in adaptation to the alpine environment: I identified four SNPs that were positively selected in the alpine populations across all five high altitude colonizations of A. arenosa. Three of these SNPs are coding and are linked together, forming a distinct alpine allele of the FAR5 protein. Using a unique crossing design based on a natural standing variation of the two identified FAR5 alleles, I prepared carriers of the non-native alleles on the genomic background of foothill and alpine populations. I then...

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