Original title: Značení \kur{Babesia divergens} kalcium-dependentní proteinkinázy 5 (BdCDPK5) metodou knock-in
Authors: ŽIROVNICKÁ, Tereza
Document type: Bachelor's theses
Year: 2024
Language: cze
Abstract: Babesia divergens is a tick-borne parasite that infects both bovines and humans, causing babesiosis. Despite its increasing impact, there are considerable limitations in understandng the key life cycle strategies of this parasite and the molecules orchestrating them. One of the crucial signaling molecules is the class of Calcium Dependent Protein Kinases (CDPKs), expected to be involved in regulating the B. divergens complex life cycle in both vertebrate and tick hosts. This thesis aims to create transgenic parasites with targeted BdCDPK5 signaling molecule using knock-in technology. In this study, we designed and successfully assembled a plasmid for knock-in targeting BdCDPK5 fused with selected reporter genes. We transfected the plasmid into B. divergens in vitro culture using nucleofection to generate transgenic parasites, with WR99210 used as a selective drug. The resulting transgenic line will serve for a detailed characterization of BdCDPK5 function throughout the B. divergens life cycle and the localization of this enzyme in the parasite cell using various microscopy techniques.
Keywords: Babesia divergens; BdCDPK5; CDPKs; genové manipulace; knock-in
Citation: ŽIROVNICKÁ, Tereza. Značení \kur{Babesia divergens} kalcium-dependentní proteinkinázy 5 (BdCDPK5) metodou knock-in. České Budějovice, 2024. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/72563

Permalink: http://www.nusl.cz/ntk/nusl-616479


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses