Original title: Stanovení viability u kultivovaných buněk pomocí fluorescence
Translated title: Measurement of cultivated cells' viability with fluorescence
Authors: Tichý, Pavel ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Document type: Bachelor's theses
Year: 2012
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií
Abstract: [cze] [eng]
Tato práce se zabývá možnostmi měření viability kultivovaných živočišných buněk pomocí jejich barvení flourescenčními indikátory. A to calceinem AM a ethidium homodimerem-1. Jsou zde také uvedeny podmínky a možnosti kultivace živočišných buněk. Dále byly vytvořeny programy v Matlabu pro detekci viability zvolenými metodami. This work is aimed to explain to possibilities of measuring viability of cultivated animal cells by staining cells with fluorescent probes. Flourescent probes described in this work are calcein AM and ethidium homodimer-1. There are also mentioned conditions and possibilities of cell cultivation. Additionaly there were created programs for cell´s viabillity detection in Matlab.
Keywords: calcein AM; cell cultivation; cell viability; ethidium homodimer-1; Flourescence microscopy; intracellular esterase activity; calcein AM; ethidium homodimer-1; Flourescenční mikroskopie; intracelulární esterázová aktivita; kultivace buněk; viabilita buněk

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/12393

Permalink: http://www.nusl.cz/ntk/nusl-596207


The record appears in these collections:
Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses