Original title: IrAM9 - a member of a thioester-containing protein family from the hard tick \kur{Ixodes ricinus}
Authors: RATHNER, Petr
Document type: Bachelor's theses
Year: 2011
Language: eng
Abstract: The thioester-containing proteins (proteins of ?2-macroglobulin family) are constituents of innate immunity, which comprise (i) universal protease inhibitors of ?2M type, (ii) C3, C4, C5 components of the complement system, (iii) insect thioester-contaning proteins (iTEPs) and (iv) macroglobulin complement related proteins (MCR). In this work, the partial structure of the thioester-containing protein from the hard tick Ixodes ricinus (IrAM9) which belong to the MCR was determined by sequencing and cloning of PCR products. The IrAM9 message is expressed in the ovaries and salivary glands of partially engorged females. Received recombinant protein was used to get polyclonal serum which was obtained by repeated rabbit immunization. The obtained antibodies were used in an attempt to detect the native protein in tick tissues by immunoblotting method.
Keywords: ?2-macroglobulin; IrAM9; Ixodes; MCR; ricinus
Citation: RATHNER, Petr. IrAM9 - a member of a thioester-containing protein family from the hard tick \kur{Ixodes ricinus}. České Budějovice, 2011. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/19687

Permalink: http://www.nusl.cz/ntk/nusl-55309


The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses