Original title: Retenční chování katecholaminů a jejich prekurzorů v HILIC podmínkách
Translated title: Retention Behaviour of Catecholamines and Their Precursors under HILIC Conditions
Authors: Tibenský, Andrej ; Kastner, Petr (advisor) ; Štěrbová, Petra (referee)
Document type: Rigorous theses
Year: 2024
Language: slo
Abstract: [eng] [cze]
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Andrej Tibenský Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Retention behaviour of catecholamines and their precursors under HILIC conditions The aim of this diploma thesis was to study retention behaviour of catecholamines and their precursors under Hydrophilic Interaction Liquid Chromatography by using spectrophotometric and fluorometric detection. 5 analytes were examined - adrenaline, noradrenaline, dopamine, L-DOPA, and tyrosine. Retention behaviour studying began by using LUNA 3 µm (PFP)2 column which possesses both RPLC and HILIC characteristics with 2 different mobile phases. The first one consisted of 25 mM acetic acid in mixture of acetonitrile and water and the second mobile phase was 25 mM acetate buffer (pH 4,50) in mixture of acetonitrile and water. Subsequently, the effect of stationary phase was studied on neutral column XBridge BEH Amide and columns Avantor ACE HILIC-A and Sequant ZIC-HILIC with ionic interactions. Mobile phases consisted of high proportion of acetonitrile (70-90 %) and their composition was the following - 25 mM acetic acid in mixture of acetonitrile and water. Then, the effect of acetic acid concentration as a mobile... Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Andrej Tibenský Vedúci diplomovej práce: PharmDr. Petr Kastner, Ph.D. Názov diplomovej práce: Retenčné správanie katecholamínov a ich prekurzorov v HILIC podmienkach Témou diplomovej práce bolo skúmanie retenčného správania katecholamínov a ich prekurzorov v HILIC podmienkach za použitia spektrofotometrickej a fluorimetrickej detekcie. Skúmaných bolo 5 analytov - adrenalín, noradrenalín, dopamín, L-DOPA a tyrozín. Najskôr bolo retenčné správanie skúmané za použitia LUNA 3 µm (PFP)2 kolóny vykazujúcej RP-LC aj HILIC charakteristiky s 2 rôznymi mobilnými fázami. Prvá bola v zložení 25 mM octovej kyseliny v zmesi acetonitrilu a vody a druhá mobilná fáza pozostávala z 25 mM acetátového pufra (pH 4,50) v zmesi acetonitrilu a vody. Následne bol skúmaný efekt stacionárnej fázy na neutrálnej kolóne XBridge BEH Amide a kolónach Avantor ACE HILIC-A a Sequant ZIC-HILIC s iónovými interakciami. Mobilné fázy obsahovali vysoký podiel acetonitrilu (70-90 %) a ich zloženie bolo nasledovné - 25 mM octovej kyseliny v zmesi acetonitrilu a vody. Ďalej sa skúmal efekt zmeny koncentrácie aditíva mobilnej fázy (octová kyselina) na retenčné správanie analytov, a to na kolónach XBridge BEH Amide a...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/187551

Permalink: http://www.nusl.cz/ntk/nusl-540691


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Rigorous theses