Discovery of General Controů Nondereprssible 5 protein interactors using proximity labeling

Potapenkova, Uliana

Usually, proteins don’t act as isolated species, but they perform their function in complexes with other proteins. Identification of protein-protein interactions (PPIs) brings insights into regulation of plant developmental processes and their response to the environmental conditions. In recent years, several methods to study PPIs were developed, since they represent the key objectives of plant system biology research. This thesis is focused on the Proximity Labeling (PL) assay in order to study GCN5 protein interactors. GCN5 is a histone acetyltransferase that plays a crucial role in plants’ defense responses to various abiotic stresses. Studies of its interactors provide valuable information on the histone acetylation mechanisms and the way plants cope with adverse conditions. Here, the advantages and disadvantages of PL assay are summarized and compared with other methods of PPIs studies. In the experimental part of this work, GCN5 was cloned and plasmid for further biotin-based proximity labeling was prepared.

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