Original title: Oligomerization state and pigment contents of algal light-harvesting proteins
Authors: MALINOVÁ, Kateřina
Document type: Master’s theses
Year: 2023
Language: eng
Abstract: [eng] [cze]
Light-harvesting antennas (LHCs) from four microalgae species (diatom Phaeodactylum tricornutum, haptophyte Emiliania huxleyi, and two eustigmatophytes Nannochloropsis oceanica and Trachydiscus minutus) were studied. LHCs were extracted and purified using sucrose gradient ultracentrifugation and ion-exchange chromatography. Oligomerization states of LHCs were assessed using two independent methods - CN-PAGE and size-exclusion chromatography. Effects of the purification on LHC pigment composition was assessed using HPLC. Light-harvesting antennas (LHCs) from four microalgae species (diatom Phaeodactylum tricornutum, haptophyte Emiliania huxleyi, and two eustigmatophytes Nannochloropsis oceanica and Trachydiscus minutus) were studied. LHCs were extracted and purified using sucrose gradient ultracentrifugation and ion-exchange chromatography. Oligomerization states of LHCs were assessed using two independent methods - CN-PAGE and size-exclusion chromatography. Effects of the purification on LHC pigment composition was assessed using HPLC.
Keywords: alga; HPLC; IEC; LHC; pigments; SEC
Citation: MALINOVÁ, Kateřina. Oligomerization state and pigment contents of algal light-harvesting proteins. České Budějovice, 2023. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/70483

Permalink: http://www.nusl.cz/ntk/nusl-538628


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses