Vliv zánětu na placentární metabolismus tryptofanu

Orbisová, Anna

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Anna Orbisová Supervisor: Prof. PharmDr. František Štaud, Ph.D. Consultant: Dr. Cilia Abad, Ph.D. Title of diploma thesis: Effect of inflammation on placental metabolism of tryptophan Maternal inflammation throughout pregnancy has been firmly linked to the development of neuropsychiatric disorders in the offspring. Furthermore, intrauterine infections, whether viral or bacterial, are widely recognized as significant risk factors for conditions like autism and schizophrenia. Tryptophan metabolism has been suggested as a plausible pathway through which maternal inflammation during gestation can hinder fetal brain development and programming. This occurs as tryptophan is metabolized through the serotonin (5-HT) pathway, producing metabolites that exert direct effects on the development of the fetal brain. In this work, using an ex vivo model, we assess the impact of bacterial (LPS) and viral (poly I:C) placental infection on the 5-HT pathway. Human term placenta explants were treated with LPS or Poly I:C for different times (4 or 18 hours). Subsequently, the impact on gene and protein expressions of the key enzymes within the 5-HT pathway, along with their functional enzymatic activities, was...

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