Original title: Porovnání vlivu bisfenolů na parentní a diferencované H9c2 buňky
Translated title: Comparison of the effect of bisphenols on parental and differentiated H9c2 cells
Authors: Macáková, Barbora ; Jirkovský, Eduard (advisor) ; Macháček, Miloslav (referee)
Document type: Master’s theses
Year: 2023
Language: cze
Abstract: [cze] [eng]
Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra farmakológie a toxikológie Študentka: Barbora Macáková Školiteľ: PharmDr. Eduard Jirkovský, Ph.D. Názov diplomovej práce: Porovnanie vplyvu bisfenolov na parentné a diferencované H9c2 bunky Bisfenoly je skupina látok vyskytujúca sa vo veľkej miere v produktoch a v životnom prostredí. Ich možná toxicita na ľudské zdravie je stále v procese študovania,pričom priamy účinok enviromentálne relevantných dávok nebol pozorovaný. Tieto endokrinné disruptory pôsobia na rôzne receptory v tele a taktiež nie je vylúčený vplyv na kardiovaskulárny systém. Najviac preskúmanou látkou je BPA, ktorý je postupne nahradzovaný svojimi analógmi, ktoré ale nie sú z veľkej časti tak preskúmané. V tejto práci sme sa zamerali na vplyv bisfenolov BP, M a S na diferenciáciu H9c2 buniek, ako modelu, s možnými negatívnymi účinkami na vývoj srdca a jeho regenerácii po poškodení. Bunková línia H9c2 majú nediferencovaný fenotyp ale môžu byť ľahko nasmerované k diferenciácii za určitých podmienok. Skúmaný bol vplyv bisfenolov na diferencované bunky a zmeny v expresii vybraných markerov (GATA4, Hand2, Tnnt2, Myog) diferenciácie (transkripčných faktorov). Dané protokoly boli sledované 9 dní a líšili sa prídavkom fetálneho hovädzieho séra (1 % alebo 10 %), all-trans... Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Barbora Macáková Supervisor: PharmDr. Eduard Jirkovský, Ph.D. Title of diploma thesis: Comparison of the effect of bisphenols on parental and differentiated H9c2 cells Bisphenol is compound largely found in products and environment around us. Its toxicity and danger for human health is still being studied, meanwhile direct influence of environmentally relevant doses was not discovered. These endocrine disruptors are affecting variousreceptorsin bodiesand effecton cardiovascularsystem isnotexcludedeither.The most extensively studied substance is BPA, which is gradually being replaced by its analogs, although these are not extensively researched to the same extent. In this work we focused on influence of bisphenol BP, M and S on differentiation of H9c2 cells, serving as a model with potential negative effects on heart development and its regeneration after damage. H9c2 cell line has an undifferentiated phenotype but can be easily directed towards differentiation under certain conditions Research was focused on impact of bisphenol on differentiated cells and its changes in the expression of selected differentiation markers (GATA4, Hand2, Tnnt2, Myog) (transcription factors). Set protocols were...
Keywords: all-trans retinoic acid; bisphenols; cardiomyoblasts; differentiation; H9c2; interference; molecular markers; serum deprivation; toxicity; all-trans retinová kyselina; bisfenoly; diferenciace; H9c2; interference; kardiomyoblasty; molekulární markery; sérová deprivace; toxicita

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/186973

Permalink: http://www.nusl.cz/ntk/nusl-537073


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses