Pohled do záhadného světa signalizace auxinu: subcellulární a tkáňově specifické aspekty v kořeni Arabidopsis thaliana

Dubey, Shiv Mani

In this dissertation, my main focus was to advance our understanding of how the plant hormone auxin regulates root growth at the sub-cellular and tissue levels in Arabidopsis thaliana. Auxin controls gene transcription through the SCFTIR1/AFB - Aux/IAA coreceptor complex. Among the TIR1/AFBs auxin receptor family members, TIR1 is strictly localized in the nucleus, while AFB1 is particularly abundant in the cytoplasm but also present in the nucleus. I confirmed the dominant role of AFB1 in controlling rapid auxin responses, such as calcium ion influx, apoplastic alkalinization and rapid root growth inhibition; processes associated with root gravitropism. I discovered a novel AFB1-dependent cytoplasmic auxin perception, and I identified the N-terminal domains of AFB1 and TIR1 crucial in determining their subcellular localization. Furthermore, my research contributed to the discovery that the root surface pH gradient on the root's longitudinal axis is not only regulated by AHA H+ -ATPases, but instead, the rapid auxin response module, comprising the AUX1 auxin influx carrier, AFB1, and the CNCG14 calcium channel, controls the apoplastic pH in the root transition zone. Further, I participated in the discovery of a deeply evolutionarily conserved rapid auxin response pathway that involves the RAF-like kinase,...

guest :: login Digital Repository
		Search		Submit		Help		About