Original title: Role of the serotonin receptor type 7 (5-HT7) in autoimmune disease
Authors: NUßBAUMER, Mia
Document type: Bachelor's theses
Year: 2023
Language: eng
Abstract: Serotonin receptor type 7 (5-HT7) displays immunomodulatory functions and is overexpressed in individuals with Crohn's disease. Here, we set out to determine 5-HT7 mode of interaction with its cognate proteins Gs and G12. A unique type of coupling, termed 'inverse coupling' is known to occur between the 5-HT7 receptor and the Gs protein. The aim of our research was to determine whether G12 can also undergo this kind of coupling. We found that the G12 protein has a surprisingly lower mobility than Gs implying a specific interaction with a membrane domain or another membrane-localized protein. For both G proteins, we were unable to detect their inverse coupling with the 5-HT7 receptor at room temperature. Our findings indicate peculiar differences in G protein mobility and emphasize the importance of temperature for studies of interactions between G proteins and G protein-coupled receptors.
Keywords: cell signaling; G protein-coupled receptor; G-protein; inflammation; microscopy; serotonin; single-molecule imaging
Citation: NUßBAUMER, Mia. Role of the serotonin receptor type 7 (5-HT7) in autoimmune disease. České Budějovice, 2023. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/66630

Permalink: http://www.nusl.cz/ntk/nusl-528966


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses