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Original title:
Vývoj UHPLC-MS/MS metody vhodné ke stanovení cabozantinibu v krevním séru
Translated title: Development of a UHPLC-MS/MS method suitable for the determination of cabozantinib in blood serum
Authors: Kleinová, Jana ; Kozlík, Petr (advisor) ; Hraníček, Jakub (referee)
Document type: Bachelor's theses
Year: 2023
Language: cze
Abstract: The aim of this Bachelor thesis was to develop a UHPLC-MS/MS method for the determination of Cabozantinib in blood serum. This method was used to monitor the release of the active substance in a model organism - the rat. First, the mass spectrometer setup was optimized, where MRM transitions for Cabozantinib and isotope-labelled Cabozantinib-D4 were found. For Cabozantinib, an MRM transition of 502.2 → 323.1 was found with optimal energy levels of Q1 = -26.0 V, CE = -40.0 V and Q2 = -22.0 V. For Cabozantinib-D4, the MRM transition of 506.2 → 327.0 was found with optimal energy levels being Q1 = -26.0 V, CE = -39.0 V and Q2 = -22 V. The method setup parameters were as follows: The chromatography column used was a Poroshell 120 EC-C18, 2.1x50 mm, 1.9 um, (Agilent Technologies). The mobile phase consisted of acetonitrile (B) and formic acid (A), the flow rate of the mobile phase was 0.350 ml/min, the analysis time was 5.5 min and the injection volume was 1 μl at gradient elution (time: 0; 0.5; 2.0; 3.0; 3.5; 4.0; 5.5 min, B: 10; 10; 60; 100; 10; 10 %). The method was linear (weighted regression 1/x2 ) with a regression coefficient equal to 0.9978, showing an excellent linearity of the method. The precision (relative standard deviation) of the method was within 14 %. The accuracy (relative error) was...
Keywords: Cabozantinib; MS; rat serum; UHPLC-MS; cabozantinib; krysí sérum; MS; UHPLC-MS
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/181750
Permalink: http://www.nusl.cz/ntk/nusl-528660
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses
Translated title: Development of a UHPLC-MS/MS method suitable for the determination of cabozantinib in blood serum
Authors: Kleinová, Jana ; Kozlík, Petr (advisor) ; Hraníček, Jakub (referee)
Document type: Bachelor's theses
Year: 2023
Language: cze
Abstract: The aim of this Bachelor thesis was to develop a UHPLC-MS/MS method for the determination of Cabozantinib in blood serum. This method was used to monitor the release of the active substance in a model organism - the rat. First, the mass spectrometer setup was optimized, where MRM transitions for Cabozantinib and isotope-labelled Cabozantinib-D4 were found. For Cabozantinib, an MRM transition of 502.2 → 323.1 was found with optimal energy levels of Q1 = -26.0 V, CE = -40.0 V and Q2 = -22.0 V. For Cabozantinib-D4, the MRM transition of 506.2 → 327.0 was found with optimal energy levels being Q1 = -26.0 V, CE = -39.0 V and Q2 = -22 V. The method setup parameters were as follows: The chromatography column used was a Poroshell 120 EC-C18, 2.1x50 mm, 1.9 um, (Agilent Technologies). The mobile phase consisted of acetonitrile (B) and formic acid (A), the flow rate of the mobile phase was 0.350 ml/min, the analysis time was 5.5 min and the injection volume was 1 μl at gradient elution (time: 0; 0.5; 2.0; 3.0; 3.5; 4.0; 5.5 min, B: 10; 10; 60; 100; 10; 10 %). The method was linear (weighted regression 1/x2 ) with a regression coefficient equal to 0.9978, showing an excellent linearity of the method. The precision (relative standard deviation) of the method was within 14 %. The accuracy (relative error) was...
Keywords: Cabozantinib; MS; rat serum; UHPLC-MS; cabozantinib; krysí sérum; MS; UHPLC-MS
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/181750
Permalink: http://www.nusl.cz/ntk/nusl-528660
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses
Record created 2023-07-09, last modified 2024-01-26