Original title: Fytochemické studie potenciálnich fytofarmak pomocí separačních metod
Translated title: Phytochemical studies of potential phytopharmaceuticals using separation methods
Authors: Dulendarova, Elizabeta Hristiyanova ; Jenčo, Jaroslav (advisor) ; Křoustková, Jana (referee)
Document type: Master’s theses
Year: 2023
Language: eng
Abstract: Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical Botany Candidate: Elizabeta Hristiyanova Dulendarova Supervisor: RNDr. Jaroslav Jenčo, Ph.D. Title of dissertation thesis: Phytochemical Studies of Potential Phytopharmaceuticals using Separation Methods The main aim of this diploma thesis was the identification of main alkaloidal components from the fraction of Papaver rhoeas L. (family Papaveraceae) alkaloidal extract - fraction PR-12-23. The introduction to the theoretical part summarizes alkaloids found in Papaver rhoeas L. and describes the separation methods used in the isolation process at the department of Phamacognosy and Pharmaceutical botany. Two main compounds within the extract were identified and separated based on HPTLC, HPLC-MS, and GC-MS chromatographic separation methods. For the subsequent fractions, the HPLC method development, optimization, and scaling up from analytical to preparative scale was done. After identification with the help of analytical HPLC and GC, the compounds were separated into fractions, isolated using preparative HPLC, and identified using NMR. The purified isolates were tested for anti-butyrylcholinesterase and cytotoxic biological activity, linked to AD and cancer, respectively. The first...
Keywords: gas chromatography; liquid chromatography; mass spectrometry; natural substances; phytopharmaceuticals; fytofarmaka; hmotnostní spektrometrie; kapalinová chromatografie; plynová chromatografie; přírodní látky

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/181139

Permalink: http://www.nusl.cz/ntk/nusl-527751


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses