Fyziologické interakce а stabilita komplexů vnitřní membrány mitochondrií.

Kafková, Anežka

Inner mitochondrial membrane (IMM) is composed of a phospholipid bilayer with a typically high ratio of protein complexes forming different complexes which execute different functions being reflected into the physiology of the whole organism. To maintain all these functions, as well as to have an ability to respond to various stress stimuli, the individual complexes of the respiratory chain interact, most likely to enhance the electron flow between these complexes, allowing the respiration steps from electron transfer from NADH to molecular oxygen to occur more efficiently. Other interactions between the complexes of the respiratory chain and other IMM complexes have been suggested. However, the underlying molecular details and functional relevance of such interactions are far from being elucidated. One of these many uncharacterised interactions involves the cytochrome c oxidase (COX), the terminal oxidase complex of the respiratory chain, and mitochondrial calcium uniporter complex (MCUC), the main gate to calcium entry into the mitochondrial matrix. Perhaps surprisingly, preliminary data from a systematic unbiased BioID analysis of MCUC subunits has consistently revealed an interaction between MCUC and COX subunits and assembly factors. In addition, SILAC-based quantitative proteomics also showed...

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