Original title: Expression and purification of Tick-borne encephalitis virus Capsid protein
Authors: SCHMELZER, Jakob
Document type: Bachelor's theses
Year: 2022
Language: eng
Abstract: Tick-borne encephalitis virus (TBEV) is an important pathogen of the family Flaviviridae with an emerging health concern. Virus causes severe neurological disorders to human population in Europa and Asia. Despite effective vaccine, there is no specific antiviral treatment till now. The capsid protein encapsules the viral positive-sense single-stranded RNA and is essential in packaging and assembling of new virions within the host. A deeper understanding of these interactions and their implications should lead to new antiviral strategies and drug design. This thesis deals with the expression and purification of the capsid protein without membrane anchor using Escherichia coli expression system.
Keywords: BL21-CodonPlus (DE3)-RIPL; Capsid protein; Cloning; Expression; Flavivirus; Immobilized Metal Affinity Chromatography; IPTG; Tick-borne encephalitis virus
Citation: SCHMELZER, Jakob. Expression and purification of Tick-borne encephalitis virus Capsid protein. České Budějovice, 2022. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/60749

Permalink: http://www.nusl.cz/ntk/nusl-520101


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses