Original title: Subcellular localization analysis of proteins non univocally located to the mitochondria of Trypanosoma brucei
Translated title: Molecular characterization of prioritized mitochondrial proteins in the protozoan parasite \kur {Trypanosoma brucei}
Authors: ESSMANN, Stina
Document type: Bachelor's theses
Year: 2021
Language: cze
Abstract: In this thesis in situ tagging by parasite transfection, followed by Western Blot and immunofluorescence microscopy was performed for the analysis of the endogenous localization of prioritized Trypanosoma brucei proteins. The prioritized non univocally located proteins had TrypTag.org annotations that corresponded or were compatible with tripartite attachment complex (TAC). For the determination of essentiality, RNAi constructs were generated by Gibson assembly molecular cloning.
Keywords: cytoplasmic; IFA; localization; mitochondrial; putative proteins; T. brucei; Western Blot; cytoplasmic; IFA; localization; mitochondrial; putative proteins; T. brucei; Western Blot
Citation: ESSMANN, Stina. Subcellular localization analysis of proteins non univocally located to the mitochondria of Trypanosoma brucei. České Budějovice, 2021. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/66329

Permalink: http://www.nusl.cz/ntk/nusl-519135


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses