Original title: Cathepsin L of Sphaerospora molnari - localisation, function and diagnostic tools
Authors: NECHVILE, Rudolf Lukas
Document type: Bachelor's theses
Year: 2022
Language: eng
Abstract: The aim of this thesis was to develop diagnostic and quantitative assays/tools based on western blotting, confocal microscopy and immunogold electron microscopy, as well as flow cytometry, to determine the localisation and potential function of the highly expressed cathepsin L of the myxozoan parasite Sphaerospora molnari (SmolCL) in its fish host, the common carp. Furthermore, a recombinant SmolCL was used in a vaccine trial to estimate its potential for raising antibodies in carp and test their immunoprotective potential towards S. molnari. This thesis provides new methodological tools for research and allows a greater understanding of myxozoan parasite-fish host interactions based on proteolytic enzymes.
Keywords: carp; cathepsin L; cnidarian parasite; cysteine protease; ELISA; flow cytometry; immunofluorescence microscopy; immunogold TEM; Myxozoa; polyclonal antibody; qPCR; recombinant protease vaccine; recombinant protein; Sphaerospora molnari; western blot
Citation: NECHVILE, Rudolf Lukas. Cathepsin L of Sphaerospora molnari - localisation, function and diagnostic tools. České Budějovice, 2022. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/66299

Permalink: http://www.nusl.cz/ntk/nusl-519128


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses