Original title:
Analysis of the dicyemid diversity using amplicon sequencing
Authors:
FLEGROVÁ, Tereza Document type: Master’s theses
Year:
2022
Language:
eng Abstract:
Using amplicon sequencing, this study reveals dicyemid diversity around the world as well as within individual cephalopod hosts. A wide number of samples of multiple species were collected to reflect true dicyemid diversity. Heterogeneity primers were designed in order to improve the sequencing performance of the Illumina platform. A complex bioinformatic pipeline was implemented to process non-overlapping reads. The species delimitation methods were used to categorize occurring dicyemid types. Using the output of implemented methods, dicyemid diversity was assessed, proposing new trends opposing the current methodology.
Keywords:
amplikonové sekvenování; dicyemida; illumina; populační diverzita Citation: FLEGROVÁ, Tereza. Analysis of the dicyemid diversity using amplicon sequencing. České Budějovice, 2022. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/59930