Original title: Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin
Authors: SELINGER, Martin
Document type: Rigorous theses
Year: 2019
Language: eng
Abstract: Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the RNA polymerase III-transcribed 5S rRNA levels.
Keywords: host response; rRNA; tick-borne encephalitis; transcriptional/translational shut-off
Citation: SELINGER, Martin. Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin. České Budějovice, 2019. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59916

Permalink: http://www.nusl.cz/ntk/nusl-515864


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Rigorous theses