Original title: Preparation of antibodies to determine the association of mitoribosomal complexes with mitochondrial membrane
Authors: ZELLNER, Laura
Document type: Bachelor's theses
Year: 2020
Language: eng
Abstract: The assembly pathways of mitoribosomes, descendants of bacterial ancestors producing proteins encoded by vestigial mitochondrial genomes, remains largely unknown. To shed light on structural features of a precursor of the highly divergent small mitoribosomal subunits (mtSSU) from Trypanosoma brucei, called mtSSU assemblosome, recently characterized by cryoEM, we raised antibodies against two assembly factors present in the complex and against two subunits of the mature mtSSU. We used the antibodies in pilot experiments to determine whether the assemblosome associates with the inner mitochondrial membrane.
Keywords: antibody production; antigen; mitochondria; mitochondrial ribosome; mitoribosome; recombinant protein; subcellular fractionation; trypanosoma brucei
Citation: ZELLNER, Laura. Preparation of antibodies to determine the association of mitoribosomal complexes with mitochondrial membrane. České Budějovice, 2020. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59549

Permalink: http://www.nusl.cz/ntk/nusl-515596


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses