guest ::
| Home > Academic theses (ETDs) > Master’s theses > Characterization of Photosystem I in the Red Alga Porphyridium purpureum |
Original title:
Characterization of Photosystem I in the Red Alga Porphyridium purpureum
Authors: KOSMAS, Panagiotis
Document type: Master’s theses
Year: 2019
Language: eng
Abstract: Photosynthesis, a fundamental biochemical process on planet Earth, has passed through several steps during the course of evolution since beginning of time. Red algae represent a primitive stage of evolution of photosynthesis in eukaryotes, hence they are important for our understanding of functioning and evolution of photosynthetic energy conversion in general. In the current work, isolation and characterization of the supramolecular complex consisting of photosystem I with three antenna complexes from the red alga Porphyridium purpureum is presented. The purification consisted of sucrose gradient centrifugation followed by anion exchange and size-exclusion chromatography. The composition of the PSI-antenna supercomplex was confirmed using electrophoresis and reverse-phase chromatography. Function of the isolated supercomplex was further investigated using time-resolved absorption and fluorescence spectroscopy.
Keywords: carotenoids; centrifugation; chlorophyll a; chloroplasts; chromatography; D1/D2 proteins; fluorescence spectroscopy; fractionation.; Light Harvesting Complex; nannochloropsis oceanica; phycobilisomes; porphyridium purpureum; psaA; SDS-gel page; sucrose band
Citation: KOSMAS, Panagiotis. Characterization of Photosystem I in the Red Alga Porphyridium purpureum. České Budějovice, 2019. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/56258
Permalink: http://www.nusl.cz/ntk/nusl-514177
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Authors: KOSMAS, Panagiotis
Document type: Master’s theses
Year: 2019
Language: eng
Abstract: Photosynthesis, a fundamental biochemical process on planet Earth, has passed through several steps during the course of evolution since beginning of time. Red algae represent a primitive stage of evolution of photosynthesis in eukaryotes, hence they are important for our understanding of functioning and evolution of photosynthetic energy conversion in general. In the current work, isolation and characterization of the supramolecular complex consisting of photosystem I with three antenna complexes from the red alga Porphyridium purpureum is presented. The purification consisted of sucrose gradient centrifugation followed by anion exchange and size-exclusion chromatography. The composition of the PSI-antenna supercomplex was confirmed using electrophoresis and reverse-phase chromatography. Function of the isolated supercomplex was further investigated using time-resolved absorption and fluorescence spectroscopy.
Keywords: carotenoids; centrifugation; chlorophyll a; chloroplasts; chromatography; D1/D2 proteins; fluorescence spectroscopy; fractionation.; Light Harvesting Complex; nannochloropsis oceanica; phycobilisomes; porphyridium purpureum; psaA; SDS-gel page; sucrose band
Citation: KOSMAS, Panagiotis. Characterization of Photosystem I in the Red Alga Porphyridium purpureum. České Budějovice, 2019. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/56258
Permalink: http://www.nusl.cz/ntk/nusl-514177
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Record created 2023-01-15, last modified 2024-03-17