Original title: Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing
Authors: KITZBERGER, Daniel
Document type: Bachelor's theses
Year: 2020
Language: eng
Abstract: CRISPR/Cas9 technique was used for genome editing of several arthropod species, but it has not yet been used on Ixodes spp. tick species. Therefore, in my work, I performed a bioinformatic analysis of the I. ricinus transcriptome and I. scapularis genome to reveal the sequence of the target gene for the CRISPR/Cas9-mediated knock-out. We prepared single guide RNA and mixed it with the Cas9 endonuclease to produce the Ribonucleoprotein complex and cleave the target sequence of the I. ricinus gene beta-galactoside alpha-2,6-sialyltransferase.
Keywords: CRISPR; CRISPR/Cas9; genome editing; Ixodes spp. ticks
Citation: KITZBERGER, Daniel. Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing. České Budějovice, 2020. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/56187

Permalink: http://www.nusl.cz/ntk/nusl-514150


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses