Original title: Hafnium Chloride, an Alternative Staining Reagent for Biological Electron Microscopy
Authors: BARANYI, Magdalena Victoria
Document type: Bachelor's theses
Year: 2020
Language: eng
Abstract: This study focuses on the staining pattern of the non-radioactive heavy metal EM stain HfCl4, used during freeze substitution specimen preparation. HfCl4 was investigated as an alternative for the prominent uranyl acetate, since uranium based materials have been placed under heavy restrictions and bans worldwide. We have found a strong HfCl4 staining pattern of microtubules in myoepithelial cells of Ixodes ricinus salivary glands. Additionally, in several samples, HfCl4 was found to completely fill the cytoplasm of the myoepithelial cells. Nonetheless, artefact formation around granulated cells was also experienced.
Keywords: cytoplasm; electron microscopy; fixation; hafnium chloride; imaging; ixodes ricinus; microtubules; myoepithelial cell; radioactive; salivary glands; staining; transmission electron microscopy; uranyl acetate
Citation: BARANYI, Magdalena Victoria. Hafnium Chloride, an Alternative Staining Reagent for Biological Electron Microscopy. České Budějovice, 2020. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/53034

Permalink: http://www.nusl.cz/ntk/nusl-513797


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses