Využití kapalinové chromatografie ve spojeni s hmotnostní detekcí pro analýzu psychoaktivních látek v netradičních matricích

Nemeškalová, Alžběta

Utilization of liquid chromatography-mass spectrometry for the analysis of psychoactive substances in unconventional matrices Abstract The growing popularity of new products used as alternatives to the traditional illegal drugs represents a current challenge for toxicological laboratories, which are required to widen the range of analytical methods and aim at new analytes and matrices. This work focuses on two types of analytes, specifically, amphetamine-like stimulants and cannabinoids, and their analysis in a variety of complex matrices. An LC-MS/MS method suitable for the analysis of amphetamine-like stimulants in newborn meconium and breast milk was developed. The sample extraction was based on the principles of salting-out assisted liquid-liquid extraction, which can be considered a potential alternative to conventional extraction techniques. The analysis was performed using a reversed-phase chromatography coupled to tandem mass spectrometry in multiple reaction monitoring mode, and the method was validated for six analytes, namely amphetamine, methamphetamine, 3,4- methylendioxymethamphetamine, mephedrone, methylone and 3,4- methylenedioxypyrovalerone. Finally, the analysis of authentic meconium and breast milk samples confirmed the applicability of the method. An LC-UV-MS/MS method for the analysis...

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