Evoluce zraku u parmy obecné (Barbus barbus)

Konvičková, Zuzana

Vertebrates inherited five visual opsin classes from their common ancestor expressed in the rod and cone cells. While many vertebrate groups reduced the number of visual pigments they possess, opsins of teleost fishes have diversified. Today, teleosts outstand among all vertebrates in the number of visual pigments and photoreceptor types. This extraordinary development was also facilitated by the teleost- specific whole genome duplication that occurred approximately 350 Mya. Here I explored teleost species that recently underwent subsequent whole genome duplication - the common barbel (Barbus barbus). This event was accompanied by hybridization (i.e., it was allopolyploidization) and resulted in 14 opsin genes found in the genome, an unusually high number even among teleosts. In this thesis, I studied the effects of such an expanded opsin repertoire on the visual abilities of the common barbel. Based on the whole genome, I have completed the reference sequences of all opsin genes and revealed their positions on the chromosomes. I analysed retinal transcriptomes and constructed developmental series of opsin expression profiles. All opsin genes were preserved functional in the genome, even though one of them (green-sensitive, named RH2-4 in this thesis) was not found in the transcriptome and showed...

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