Original title: Comparison of the Covalent Laccase Immobilization at Amino- and Carboxylfunctionalized Mesoporous Silica, Glassy Carbon, and Graphite Powders using Different Coupling Agents for Optimal Biosensor Preparation
Authors: Tvorynska, Sofiia ; Barek, J. ; Josypčuk, Bohdan
Document type: Papers
Conference/Event: Moderní Elektrochemické Metody /40./, Jetřichovice (CZ), 20211108
Year: 2021
Language: eng
Abstract: In order to find the most suitable immobilization protocol, a comparison of three strategies\nbased on the application of –NH2 and –COOH functionalized supports with the different\nactivation agents (glutaraldehyde and carbodiimide) have been conducted for the covalent\nenzyme (laccase) attachment. Two kinds of the supports, namely mesoporous silica (SBA−15,\nMCM−41) and carbonaceous (glassy carbon, graphite) powders, have been used. It was found\nthat a biosensor consisted of tubular detector of silver solid amalgam as a working electrode\nand the enzymatic mini-reactor with laccase covalently attached to glutaraldehyde activated\n–NH2 functionalized MCM−41 shows the best results regarding sensitivity and stability for\ndopamine detection.
Keywords: Covalent enzyme immobilization; Laccase; Support surface functionalization
Project no.: GA20-01589S (CEP), GA20-07350S (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: Sborník přednášek mezinárodní odborné konference XL. Moderní Elektrochemické Metody, ISBN 978-80-905221-8-3

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0325150

Permalink: http://www.nusl.cz/ntk/nusl-508262

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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers
 Record created 2022-09-28, last modified 2024-04-15

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