Original title: Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies
Authors: Tvorynska, Sofiia ; Barek, J. ; Josypčuk, Bohdan
Document type: Papers
Conference/Event: ISC Modern Analytical Chemistry /17./, Prague (CZ), 20210916
Year: 2021
Language: eng
Abstract: In this work, a promising combination of a biosensor based on the\nenzymatic mini-reactor with the detection principle of four-electron\nreduction of the consumed oxygen at highly negative potential has\nbeen developed for uric acid determination using flow injection\nanalysis. The construction of the biosensor provides a spatial\nsegregation of the biorecognition (uricase-based mini-reactor) and\ndetection (tubular detector of silver solid amalgam (TD-p-AgSA))\nparts. To find out the most appropriate enzyme immobilization\nprotocol, three different strategies of the covalent attachment for\nuricase from Bacillus fastidiosus have been compared. It was found\nthat the biosensor with the mini-reactor based on the covalent\nattachment of uricase via glutaraldehyde to -NH2 functionalized\nmesoporous silica powder MCM-41 showed extremely high stability\n(>1 year) and reusability (at least 600 measurements) The biosensor's\npractical applicability was confirmed by successful determination\nof uric acid in human urine.
Keywords: amalgam electrode; amperometric biosensor; uric acid
Project no.: GA20-01589S (CEP)
Funding provider: GA ČR
Host item entry: Proceedings of the 17th ISC Modern Analytical Chemistry, ISBN 978-80-7444-089-2

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0322725

Permalink: http://www.nusl.cz/ntk/nusl-508174


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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers