Original title: Vývoj ex vivo plicního perfuzního systému pro animální modely
Translated title: Development of an ex vivo lung perfusion system focusing on the preservation of fresh animal lungs for experiments and storage
Authors: Mesíková, Klaudia ; Forjan, Mathias (referee) ; Paštěka, Richard (advisor)
Document type: Master’s theses
Year: 2022
Language: eng
Publisher: Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií
Abstract: A mechanical combined lung model is a type of model used in human breathing simulation. The biggest currency of the model is a high similarity with the human lungs. In order to work with the animal lungs for a longer time and so follow the principles of the 3Rs, a perfusion system is involved in the procedure. The perfusion system filled with a chosen perfusate solution is responsible to prolong the period in which the animal lungs are viable for experiments and storage in the ex-vivo environment. The development of the properly functioning perfusion system is based on the several components included in the process. Choosing the right solution for the perfusion of the inner environment of the lungs is one of the most important things that need to be taken into account. The roller pump is considered the drive motor of the system. Pressure and flow sensors are responsible for monitoring the process parameters that could describe the functionality and the ability to preserve the animal lungs in the ex-vivo environment. The validation of the developed system by using the fresh animal lungs is a part of the thesis as well as the checking procedure of the solution’s influence with the time of the storage. The perfusion system was successfully created and tested. The pressure and flow parameters gained during the measurement were compared while using the saline solution, the Ringer’s solution, and Histofix in the system. The compliance parameter of the lungs were been monitored during the perfusion as well as during the storage with the aim to determine the behaviour of the preserved lungs with the time and the impact of the chosen solution on it. Compliance initially decreased and then stabilized at a certain value throughout the storage period. For the perfusion with the saline and Ringer’s solution, it dropped by one-third. For Histofix preservation, the drop was by half of the initial compliance. The preservation time without the presence of the tissue necrosis was 120 hours using the Saline solution, 240 hours using the Ringer’s solution, and at least 268 hours using Histofix. The perfusion system could further be used in medical research and make a positive aspect in terms of less consumption of the animal organs for experimental purposes in various fields of the research. For future research, the improvement of the perfusion system and solution composition to ensure even longer preservation is welcomed.
Keywords: Animal lungs; Microcontroller; Perfusate solution; Perfusion system; Preservation; Animal lungs; Microcontroller; Perfusate solution; Perfusion system; Preservation

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/208147

Permalink: http://www.nusl.cz/ntk/nusl-506175


The record appears in these collections:
Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Master’s theses