Original title: Identifikace a biochemická charakterizace lektinů v hemolymfě tří druhů klíšťat rodu \kur{Rhipicephalus}
Translated title: Identification and biochemical characterization of lectins in the hemolymph of three species of tick in the genus \kur{Rhipicephalus}
Authors: FIŠER, Miroslav
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: Lectins are tissue specific carbohydrate binding proteins with possible functions in invertebrate immunity and pathogen transmission. The main goal of this study was to identify hemolymph lectins in three different tick species. Three proteins with molecular weights of 58 kDa, 75 kDa and 180 kDa were detected in all investigated species using antibodies directed against hemagglutination activity of Rhipicephalus appendiculatus hemolymph. These proteins were characterized by biochemical methods such as Schiff/periodate staining, lectin blotting, enzymatic deglycosylation, hemagglutination analysis, immunoblotting, and mass spectrometry.
Keywords: glycosylation; hemolymph; lectin; Rhipicephalus; tick; glykosylace; hemolymfa; klíště; lektin; Rhipicephalus
Citation: FIŠER, Miroslav. Identifikace a biochemická charakterizace lektinů v hemolymfě tří druhů klíšťat rodu \kur{Rhipicephalus}. České Budějovice, 2009. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/6335

Permalink: http://www.nusl.cz/ntk/nusl-49953


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses