Original title: Vliv Glycopyrronium Bromide a Tiotropium Bromide na proteiny těsných spojů v hematoencefalické bariéře in vitro
Translated title: The effects of Glycopyrronium Bromide and Tiotropium Bromide on tight junctional proteins of blood-brain barrier mimicking cells and their transport across a blood-brain barrier in vitro model
Authors: Vaněček, Václav ; Štaud, František (advisor) ; Čečková, Martina (referee)
Document type: Master’s theses
Year: 2007
Language: eng
Abstract: [eng] [cze]
In this thesis, we present information on the effect of glycopyrronium and tiotropium on tight junctions in the blood-brain barrier. We also studied transport of these substances through an in vitro model of blood-brain barrier. Using ELISA method, we confirmed the effect of glycopyrronium on the expression of ocludin, ICAM-1 and claudine-1 and the effect of tiotropium on the expression of claudine-1. By transport studies across the ECV304 cell monolayers, we conclude that both substances have the potential to migrate through the blood-brain barrier. V této diplomové práci prezentujeme informace o vlivu glykopyronia a tiotropia na těsné spoje hematoencefalické bariéry. Dále jsme se zabývali transportem těchto látek přes in vitro model hematoencefalické bariéry. Metodami ELISA jsme potvrdili vliv glykopyronia na expresi okludinu, ICAM-1 a claudinu-1 a vliv tiotropia na expresi claudinu-1. Transportními studiemi přes buněčné monovrstvy ECV304 jsme došli k závěru, že obě sledované látky mají potenciál přestupovat hematoencefalickou bariéru.

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/10646

Permalink: http://www.nusl.cz/ntk/nusl-492974


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses