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Original title:
Role genu WT1 v normální hematopoese a leukemogenese
Translated title: The Role of WT1 gene in normal hematopoiesis and leukemogenesis
Authors: Kramarzová, Karolina ; Trka, Jan (advisor) ; Zadražil, Stanislav (referee)
Document type: Master’s theses
Year: 2008
Language: cze
Abstract: 6 Abstract The Wilms' tumor gene is highly expressed in a large proportion of human acute leukemias and other hematological malignancies. It is thought to play an important role in human hematpoiesis and leukemogenesis. Despite the large number of studies, WT1 expression patterns and its clinical significance in acute myeloid leukemia remain still controversial. To investigate the prognostic relevance of initial WT1 expression and its usefulness as a marker for minimal residual disease, we have analysed 66 bone marrow samples from newly diagnosed AML patients. RQ-RT-PCR for absolute quantification of total WT1 was designed according to Europe Against Cancer Program. In 82 % of samples we detected a higher expression of WT1 compared to normal healthy donors or patients with acute lymphoblastic leukemia (p<0.0001). We did not find any correlation between initial WT1 expression level and age or sex. Patients with FAB M3 subtype showed significantly higher WT1 levels than other subtypes, especially M5 (p<0.001). We found a strikingly high WT1 expression in standard risk group patients compared to high risk group (p<0.0007). Children with FAB/cytogenetical favorable prognostic factors have high WT1 expression, while there was no relation between WT1 levels at diagnosis and day 15 BM response. We did not find any...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/5514
Permalink: http://www.nusl.cz/ntk/nusl-484720
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Translated title: The Role of WT1 gene in normal hematopoiesis and leukemogenesis
Authors: Kramarzová, Karolina ; Trka, Jan (advisor) ; Zadražil, Stanislav (referee)
Document type: Master’s theses
Year: 2008
Language: cze
Abstract: 6 Abstract The Wilms' tumor gene is highly expressed in a large proportion of human acute leukemias and other hematological malignancies. It is thought to play an important role in human hematpoiesis and leukemogenesis. Despite the large number of studies, WT1 expression patterns and its clinical significance in acute myeloid leukemia remain still controversial. To investigate the prognostic relevance of initial WT1 expression and its usefulness as a marker for minimal residual disease, we have analysed 66 bone marrow samples from newly diagnosed AML patients. RQ-RT-PCR for absolute quantification of total WT1 was designed according to Europe Against Cancer Program. In 82 % of samples we detected a higher expression of WT1 compared to normal healthy donors or patients with acute lymphoblastic leukemia (p<0.0001). We did not find any correlation between initial WT1 expression level and age or sex. Patients with FAB M3 subtype showed significantly higher WT1 levels than other subtypes, especially M5 (p<0.001). We found a strikingly high WT1 expression in standard risk group patients compared to high risk group (p<0.0007). Children with FAB/cytogenetical favorable prognostic factors have high WT1 expression, while there was no relation between WT1 levels at diagnosis and day 15 BM response. We did not find any...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/5514
Permalink: http://www.nusl.cz/ntk/nusl-484720
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Record created 2022-05-08, last modified 2022-05-08