Original title: Rezistence k antibiotikům udílená proteiny ARE podrodiny ABC proteinů
Translated title: Antibiotic resistance conferred by members of ARE subfamily of ABC proteins
Authors: Veselá, Ludmila ; Balíková Novotná, Gabriela (advisor) ; Borčin, Kateřina (referee)
Document type: Bachelor's theses
Year: 2012
Language: cze
Abstract: [cze] [eng]
Hlavním tématem této práce je ARE podrodina ABC transportérů. Důležitost proteinů této podrodiny spočívá v tom, že udělují rezistenci k několika skupinám klinicky důležitých antibiotik: makrolidům, linkosamidům, streptograminům a pleuromutilinům a to u významných patogenů jakým je např. Staphylococcus aureus. Na rozdíl od klasických ABC transportérů struktura ARE proteinů postrádá transmembránovou doménu (TMD) a prozatím nebyl nalezen ani interagující transmembránový protein. Z toho důvodu není jasný mechanismus udílení rezistence. V práci diskutujeme obě navrhované hypotézy mechanismu funkce těchto proteinů. První je aktivní efflux antibiotika ven z bakterie. Druhá hypotéza spočívá v uvolnění antibiotika z jeho vazebného místa, které je iniciované ARE proteiny, a jeho následná pasivní difuse ven z buňky. Klíčová slova: ABC proteiny, ARE proteiny, rezistence, MLS, Vga The main topic of this thesis is the ARE subfamily of ABC transporters. The importance of the proteins of this subfamily lies in the fact that they confer resistance to several classes of clinically important antibiotics: macrolides, lincosamides, streptogramines and pleuromutilines and they do it in significant pathogens, as for example Staphylococcus aureus. Compared to canonical ABC transporters, the structure of ABC proteins lacks the transmembrane domain (TMD) and so far, there where not even found an integrating transmembrane protein. Due to these facts, the mechanism of resistance conferred by these proteins remains unclear. In the thesis, both suggested hypotheses of the mechanism of how these proteins work are discussed. The first hypothesis presumes the active efflux of antibiotics out of the bacteria. The second hypothesis suggests release of antibiotic from its binding site initiated by ARE proteins, followed by its passive diffusion out of the cell. Keywords: ABC proteins, ARE proteins, resistance, MLS, Vga
Keywords: ABC proteins; antibiotic resistance; lincosamides; macrolides; resistance mechanisms; streptogramins; ABC proteiny; linkosamidy; makrolidy; mechanismy rezistence; rezistence k antibiotikům; streptograminy

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/44871

Permalink: http://www.nusl.cz/ntk/nusl-484127


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses