Original title:
Functional analysis of the YCF 45 gene in procyclic \kur{Trypanosoma brucei}
Translated title:
Functional analysis of the YCF 45 gene in procyclic \kur{Trypanosoma brucei}
Authors:
TÝČ, Jiří Document type: Bachelor's theses
Year:
2008
Language:
cze Abstract:
In this study was shown by RNA interference that YCF 45 gene of putative chloroplast origin is not essential for surviving of procyclic Trypanosoma brucei. Three different construct bearing HA-tagged YCF 45 protein that differs on their N-terminus were generated to reveal the localization of the protein in the cell. Spliced leader acceptor site of YCF 45 mRNA was identified and in comparison to annotated data new start codon was proposed. Fylogeny tree was made to shed some light on the origin of this gene.
Keywords:
chloroplast; HA-tag; kinetoplastids; RNA interference Citation: TÝČ, Jiří. Functional analysis of the YCF 45 gene in procyclic \kur{Trypanosoma brucei}. České Budějovice, 2008. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/9769