Original title: Studium vlivu posttranslačních modifikací na strukturu proteinu za využití \kur{Leishmania tarentolae} jako expresního systému: vývoj metodiky isotopického značení proteinů
Translated title: Influence of posttranslation modifications on protein structure: development of methodology for isotopic labeling of proteins in \kur{Leishmania tarentolae} expression system
Authors: MATULOVÁ, Jana
Document type: Bachelor's theses
Year: 2007
Language: cze
Abstract: We have established a cost efficient approach for isotopic labeling of proteins using Leishmania tarentolae expression system. The method is based on cultivation L. tarentolae in a rich complex (BHI) medium supplemented with labeled amino acid(s). This approach leads to low level isotope enrichment, which implies the decreased sensitivity of NMR experiment. In the same time this approach leads to a two to three fold increase of the yield of the recombinant protein. Importantly, it leads to 5 {--} 10 fold reduction of the overall costs as compared to the recently established protocol (Niculae et al., 2006). In parallel, we have attempted to estimate the suitability of L. taretolae for in-cell NMR spectroscopy.
Citation: MATULOVÁ, Jana. Studium vlivu posttranslačních modifikací na strukturu proteinu za využití \kur{Leishmania tarentolae} jako expresního systému: vývoj metodiky isotopického značení proteinů. České Budějovice, 2007. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/4006

Permalink: http://www.nusl.cz/ntk/nusl-46912


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses