Original title: Konformace adenylátcyklázového toxinu Bordetella pertussis.
Translated title: Conformation of the adenylate cyclase toxin of Bordetella pertussis.
Authors: Motlová, Lucia ; Fišer, Radovan (advisor) ; Obšil, Tomáš (referee) ; Holoubek, Aleš (referee)
Document type: Doctoral theses
Year: 2021
Language: cze
Abstract: [cze] [eng]
Tato práce se zabývá uspořádáním RTX ("Repeats in ToXin") domén vybraných RTX toxinů a vlivem těchto struktur především na balení toxinů do nativní struktury jejich sekreci a. Pro strukturní analýzu byly vybrány RTX domény ApxI (Actinobacillus pleuropneumoniae-RTX- toxin I) z bakterie Actinobacillus pleuropneumoniae, HlyA (Alfa-hemolysin) z bakterie Escherichia coli a LtxA (Leukotoxin A) z bakterie Aggregatibacter actinomycetemcomitans a bloky 4 a 5 RTX domény CyaA (adenylátcyklázový toxin) z bakterie Bordetella pertussis. Podařilo se získat a charakterizovat struktury RTX domény Ltxa A a RTX bloky 5 a 4-5 CyaA. Na základě SAXS (maloúhlový RTG rozptyl) modelu, dříve vyřešených struktur RTX proteinů a zde prezentovaných struktur byly vytvořeny dva modely celé rozsáhlé RTX domény CyaA. This work is focused on the RTX (Repeats in ToXin) domains structure of selected RTX toxins and its impact on secretion and protein folding. The structural analysis included RTX domains of ApxI (Actinobacillus pleuropneumoniae-RTX-toxin I) from Actinobacillus pleuropneumoniae, HlyA (Alfa-hemolysin) from Escherichia coli and LtxA (Leukotoxin A) from Aggregatibacter actinomycetemcomitans and blocs 4 a 5 RTX domain CyaA (adenylate cyclase toxin) from Bordetella pertussis. The structures of LtxA RTX domain and CyaA RTX blocs 4 and 5 were obtained and characterized. Two models of CyaA RTX domain were built based on SAXS (Small Angle X-ray Scattering) model, previously solved RTX structures and RTX structures presented here.
Keywords: adenylate cyclase toxin; black lipid membranes; Bordetella; fluorescence; RTX toxin; adenylátcyklázový toxin; Bordetella; fluorescence; RTX toxin; černé lipidové membrány

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/170903

Permalink: http://www.nusl.cz/ntk/nusl-455022


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses