Original title: My_Umi_Tool: Processing Of Unique Molecular Identifiers Without Mapping To A Reference Genome
Authors: Barilíková, Lujza
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: The presented paper describes a novel algorithm for processing unique molecular identifiers (UMIs) without mapping to a reference genome. For the past few years, there has been a rapid rise in the use of sequencing technologies and progress in bioinformatics tools. However, available tools for processing UMIs are usually relative computationally demanding and time-consuming which led us to design and develop a new algorithm as well as to implement some available software to provide an effective estimation of the total number of unique molecules. My_UMI_tool performs four major steps: preprocessing of an input file, clustering by UMIs, clustering by sequence similarity, and marking duplicates to generate final file.
Keywords: next-generation sequencing (NGS); PCR error; unique molecular identifiers (UMIs)
Host item entry: Proceedings II of the 26st Conference STUDENT EEICT 2020: Selected papers, ISBN 978-80-214-5868-0

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/200625

Permalink: http://www.nusl.cz/ntk/nusl-447677


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