Original title: Reproducible Analytical Pipeline For Using Raw Rna-Seq Data From Non-Model Organisms
Authors: Schwarzerová, Jana
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: Current biotechnological research of bacterial or archaeal genomes has huge potential due to the use of the next generation sequencing (NGS) platforms. NGS era unravelled huge analysis data for sufficiency description microorganisms with ecology potential in future. Nowadays, efforts lie in creating comprehensive pipelines that can be used for pre-processing analysis to enable effective following steps of high throughput data processing. This paper deals with design of data analysis pipeline for using raw RNA-Seq data that was applied to the Clostridium beijerinckii NRRL B-598. The bacterium is typical performer in the field of biofuels production thanks to its ability to produce butanol. Unfortunately, it is non-model organism as many other microorganisms which can be of great potential from ecological point of view. The proposed pipeline offers to take necessary steps in initial data processing that produces data of comparable quality to widely studied model organisms. Therefore, it can be combined with following pipelines for gene regulatory network inference, which was up to date matter of non-model organisms.
Keywords: Clostridium beijerinckii NRRL B-598; Next generation sequencing; RNA-Seq; Transcriptome
Host item entry: Proceedings I of the 26st Conference STUDENT EEICT 2020: General papers, ISBN 978-80-214-5867-3

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/200564

Permalink: http://www.nusl.cz/ntk/nusl-447616


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