Original title: Identifikace nekódující RNA u Clostridium beijerinckii NRRL B-598 pomocí RNA-Seq dat
Translated title: Identification of non-coding RNAs of Clostridium beijerinckii NRRL B-598 using RNA-Seq data
Authors: Pomykalová, Barbora ; Sedlář, Karel (referee) ; Jurečková, Kateřina (advisor)
Document type: Bachelor's theses
Year: 2021
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií
Abstract: [cze] [eng]
Tato bakalářská práce obsahuje stručný úvod do problematiky nekódujících malých RNA u bakterií (sRNA). Zaměřuje se na jejich vlastnosti a funkce v organismu a to konktrétně pro bakterii Clostridium beijerinckii NRRL B-598. Dále obsahuje popis laboratorních metod pro stanovení genové exprese a navrhuje postup pro identifikaci malých nekódujících RNA z dat získaných metodou RNA-Seq, pro zkoumanou bakterii Clostridium beijerinckii NRRL B-598. V neposlední řadě dochází k implementaci navrženého postupu v prostředí MATLAB a zhodnocení výsledků získaných touto metodou. This bachelor thesis contains short introduction into bacterial small non-coding RNA problematic. It is oriented on their features and functions in organisms, especially in bacteria Clostridium beijerinckii NRRL B-598. Bachelor thesis also contains description of various laboratory methods for gene expression determination and suggests a detection method for small non-coding RNA in bacteria Clostridium beijerinckii NRRL B-598. Suggested method works with data, which were obtained by RNA-Seq technology. Within the framework of the bachelor thesis was suggested method implemented in programming and numeric computing platform MATLAB and its results were discussed.
Keywords: bacteria; detection; gene expression; MATLAB; RNA; RNA-Seq; sequencing; sRNA; bakterie; detekce; genová exprese; MATLAB; RNA; RNA-Seq; sekvenování; sRNA

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/198497

Permalink: http://www.nusl.cz/ntk/nusl-443910


The record appears in these collections:
Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses