Funkcionalizace bezbuněčných nosičů pro kostní tkáňové inženýrství pomocí bioaktivních látek

Lukášová, Věra

There are good prospects for bone tissue engineering and therefore researcher is aimed towards the development of cell-free scaffolds. A cell-free scaffold serves as a temporal filling for critical size defects that do not heal spontaneously. Nevertheless, a suitable scaffold composition is yet to be discovered. Moreover, modifications of cell-free scaffolds with a drug delivery system activate the internal healing capacity. Platelets occur in the healing cascade as a natural source of growth factors (GFs), chemokines and cytokines. This autologous source of bioactive compounds enables the substitution of synthetic GFs. The aim of this thesis is to develop a bioactive cell-free scaffold with a drug delivery system supporting the physiological healing of bone defects. The centrifugal spinning method was used to produce nanofibrous poly-ε-caprolactone (PCL) scaffolds. PCL scaffolds were functionalized with different platelet concentrations. Bioactive compounds released from activated platelets were trapped within the formed fibrin net, enabling their gradual release. Improved metabolic activity, proliferation and alkaline phosphatase activity of MG-63 cells and human mesenchymal stem cells (hMSCs) were detected. The release of compounds lasted for two weeks and nearly reached the plateau phase,...

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