Original title: Production of polyclonal antibodies against recombinant potato virus X coat protein
Translated title: Produkce polyklonálních protilátek proti rekombinantnímu proteinu X viru bramboru
Authors: Čeřovská, Noemi ; Plchová, Helena ; Moravec, Tomáš ; Filigarová, Marie ; Dědič, P.
Document type: Papers
Conference/Event: Funkční genomika a proteomika ve šlechtění rostlin, Křtiny (CZ), 2008-09-09 / 2008-09-10
Year: 2008
Language: eng
Abstract: [eng] [cze]
The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX in laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot. Gen kódující obalový protein (CP) X viru bramboru (PVX) byl klónován v expresním vektoru pMPM-4AΩ . PVX CP byl exprimován v Escherichia coli a byl použit pro produkci králičích polyklonálních protilátek.Získaná antiséra a protilátky byly testovány na přítomnost PVX v hostitelských rostlinách Nicotiana tabacum cv. Petit Havana SR 1 a N. benthamiana a v různých kultivarech přírodního hostitele Solanum tuberosum Protilátky byly specifické pro PVX a použitelné pro detekci širokého spektra izolátů PVX při použití metody IPTA ELISA a Western blotu.
Keywords: polyclonal antibodies; recombinant coat protein; Y potato virus
Project no.: CEZ:AV0Z50380511 (CEP), 1M06030 (CEP)
Funding provider: GA MŠk
Host item entry: proceedings of conferences, ISBN 978-80-7375-234-7

Institution: Institute of Experimental Botany AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0173181

Permalink: http://www.nusl.cz/ntk/nusl-40295


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Research > Institutes ASCR > Institute of Experimental Botany
Conference materials > Papers