Original title: Expression and purification of adhesive recombinant proteins, sericin 2 and salivary gland secretion 3
Authors: VU, Trang Thanh
Document type: Bachelor's theses
Year: 2019
Language: eng
Abstract: Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and partially characterised. The identity of the expressed recombinant proteins was verified by mass spectroscopy. The recombinant proteins were tested for their ability to coat hydrophobic surfaces and sequentially serve as a substrate for the attachment of cells in tissue culture. The quality of the recombinant protein surface coating was examined by scanning electron microscopy. The results show that further optimisation for the purification, solubilization and refolding of these recombinant proteins is needed to incorporate their potential as biomaterials in the future.
Keywords: bombyx; drosophila; expression; proteins; recombinant
Citation: VU, Trang Thanh. Expression and purification of adhesive recombinant proteins, sericin 2 and salivary gland secretion 3. České Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/55703

Permalink: http://www.nusl.cz/ntk/nusl-399120


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses