Original title: Detection of Single Nucleotide Polymorphisms Using Selective Incorporation of Biotin in DNA Strand and Subsequent Enzymatic Detection at Pencil Electrode
Authors: Plucnara, Medard ; Ecsin, E. ; Erdem, A. ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní Elektrochemické Metody /34./, Jetřichovice (CZ), 2014-05-19 / 2014-05-23
Year: 2014
Language: eng
Abstract: Enzyme-linked electrochemical assay using DNA labeling by biotin followed by binding of enzyme-streptavidin conjugates has been found to be a potent tool for DNA diagnostic. This approach brings a special advantage of signal amplification due to the fact, that only very small number of enzymatic labels can produce a number of molecules of an electrochemically detectable product from an inactive substrate to obtain sufficiently strong signal. A new way of using of this technique combined with selective primer extension reaction designed for the detection of single nucleotide polymorphism is presented here. The assay was combined with measurements at a pencil graphite electrode, which is a very practical tool for potential clinical applications due to its cheapness and disposability.
Keywords: Enzymatic labeling; Pencil graphite electrode; Single nucleotide polymorphism
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXIV. Moderní elektrochemické metody sborník přednášek, ISBN 978-80-905221-2-1

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297937

Permalink: http://www.nusl.cz/ntk/nusl-396440


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers