Original title: Study on the Electrophoretic Behaviour of Short Oligodeoxyribonucleotides in Fused Silica Capillaries. A Preliminary Communication
Authors: Vítová, Lada ; Fojta, Miroslav ; Vespalec, Radim
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: Effects of background electrolyte pH, composition and concentration on the electrophoretic transport of short oligodeoxyribonucleotides (ODNs) in fused silica capillaries have been investigated in the presented study. Mobilities and separation efficiencies of ODNs peaks varied with all three background electrolyte characteristics. Just changes in the background electrolyte characteristics and acid-base properties of ODNs are not sufficient for a systematic explanation of the observed variations. Despite the indicated complexity of ODNs electrophoretic behaviour, we show that very high separation efficiencies of ODNs peaks can be achieved in background electrolytes of physiological pH.
Keywords: capillary electrophoresis; nucleotides; oligonucleotides; separation
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297910

Permalink: http://www.nusl.cz/ntk/nusl-396431


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Conference materials > Papers