Original title: Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads
Authors: Plucnara, Medard ; Havran, Luděk ; Ecsin, E. ; Erdem, A. ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year: 2016
Language: eng
Abstract: Novel approach of SNP detection in model synthetic oligonucleotides is described.This assay requires only intrinsic electrochemical properties of DNA and except of incorporation of biotin affinity tag, no any labeling or any other modification step is needed. Very good resolution for different variants of polymorphism as well as sufficiently low detection limits were achieved.In combination with using pencil graphited electrode this assay could be very simple and cheap tool for SNP typing.
Keywords: copper ions; Label-free detection; surface-plasmon resonance
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297885

Permalink: http://www.nusl.cz/ntk/nusl-396407


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers