Original title:
Amalgam Electrodes Pretreatment and Electrochemical Analysis of DNA
Authors:
Daňhel, Aleš ; Fojta, Miroslav Document type: Papers Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year:
2016
Language:
eng Abstract:
Mercury meniscus silver solid amalgam electode (m-AgSAE) represents the most suitable and perspective alternative to mercury electrodes which excel in sensitivity to biopolymers and their structural changes. This work briefly summarizes factors influencing electrochemical analysis of DNA at mercury and amalgam electrodes and it is mainly devoted to development of suitable pretreatment procedure, which could securte repeatable and reliable measurements of DNA at m-AgSAes. The results are essential for further utilization of amalgam electrodes in developement of biosensors, since DNA samples are very strongly adsorbed at the electrodes, and none pretreatment procedure was found to be successful in their complete desorption.
Keywords:
double-helical polynucleotides; electrically charged surfaces; mercury-electrode; silver amalgam Project no.: GA16-01625S (CEP) Funding provider: GA ČR Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5
Institution: Institute of Biophysics AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0297870