Original title: Analysis of Products of Nontemplate Enzymatic Synthesis of DNA Oligonucleotides using Voltammetric Methods
Authors: Hermanová, Monika ; Havranová-Vidláková, Pavlína ; Ondráčková, Anna ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /38./, Jetřichovice (CZ), 20180521
Year: 2018
Language: eng
Abstract: Nontemplate DNA synthesis is a feature specific for a special type of DNA polymerase, terminal deoxynucleotidyl transferase (TdT). This enzyme is able. to randomly add nucleotides to a sintde-stranded DNA primer, provided the 3'OH end of the primer is accessible, Here we show that voltammetric analysis based on hanging mercury drop electrode (EPODE) and pyrolytic graphite electrode (PGE) enables to study this nontemplate DNA synthesis.
Keywords: Hanging mercury drop electrode; Nontemplete DNA synthesis; Pyrolytic graphite electrode
Project no.: GBP206/12/G151 (CEP), EF15_003/0000477
Funding provider: GA ČR, GA MŠk
Host item entry: XXXVIII. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-6-9

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297794

Permalink: http://www.nusl.cz/ntk/nusl-396387


The record appears in these collections:
Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers