Original title: Deactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria \kur{Rhodobacter sphaeroides}
Authors: ŠÍMOVÁ, Ivana
Document type: Master’s theses
Year: 2019
Language: eng
Abstract: The main task of this thesis is to find the ICT activator of a carotenoid spheroidenone in the pigment-protein complex LH1 of Rhodobacter sphaeroides. The ICT state is characterized by a red-shifted positive transient absorption signal around 750 - 800 nm. We hypothesize that the presence of the ICT state is associated with the pigment-protein interaction of a carotenoid keto group and specific amino acid in the protein. To perform the experiment, four mutant complexes with amino acids substitutions, which should result in decreased intensity of the ICT signal due to a weaker or missing pigment-protein interaction, were obtained and measured using femtosecond time resolved pump-probe spectroscopy.
Keywords: carotenoid; ICT; pump-probe; spectroscopy
Citation: ŠÍMOVÁ, Ivana. Deactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria \kur{Rhodobacter sphaeroides}. České Budějovice, 2019. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/53098

Permalink: http://www.nusl.cz/ntk/nusl-394544


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses