Regulace morfogenních procesů u brambor - role sacharidového metabolismu

Ševčíková, Hana

Potato (Solanum tuberosum) tuberization is a complex, strictly regulated morphogenic process. Since potato is one of the most important crops in the world, understanding the regulation of this process is gaining in importance not only from a theoretical but also from a practical point of view. This work focuses on the role of sugars, which can serve not only as an energy and building material sources, but also as an important signal regulating many developmental processes including the tuberization. As a primary experimental material potato cv. Lada (WT) and its spontaneously tuberizing (ST) mutant was used. Since the previous results with ST plants suggested altered carbohydrate partitioning between plant organs and significant difference in total carbohydrate contents between ST plants and the WT (Fischer et al. 2008), in the first manuscript presented, the aim was to examine in detail the sugar metabolism of ST plants grown in vitro mixotrophically (MT) and photoautotrophically (PA). We observed changes in soluble carbohydrate allocation and starch deposition, favouring basal stem part in ST. Even thought, gibberellins (GAs) are considered to be the main tuberization inhibitors, ST potato plants had surprisingly high GAs levels. The determination of tuber-inducing marker gene expressions...

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