Original title:
Localization of the F1-ATP Synthase Subunit alpha and its N' and C' Terminal Fragments in Trypanosoma brucei cells
Authors:
LEHMAYER, Leonie Document type: Bachelor's theses
Year:
2018
Language:
eng Abstract:
[eng][cze] In this thesis, we explored if the proteolytically excised octapeptide from the Trypanosoma brucei F1-ATP synthase subunit can act as a mitochondrial targeting sequence. Three procyclic 29.13 T. brucei cell lines were generated to express tagged variants of the subunit. We then determined where these heterologous proteins were localized within the parasite. This preliminary data provides insights about where the subunit proteolytic cleavage events occur within the cell. In this thesis, we explored if the proteolytically excised octapeptide from the Trypanosoma brucei F1-ATP synthase subunit alpha can act as a mitochondrial targeting sequence. Three procyclic 29.13 T. brucei cell lines were generated to express tagged variants of the alpha subunit. We then determined where these heterologous proteins were localized within the parasite. This preliminary data provides insights about where the alpha subunit proteolytic cleavage events occur within the cell. Citation: LEHMAYER, Leonie. Localization of the F1-ATP Synthase Subunit alpha and its N' and C' Terminal Fragments in Trypanosoma brucei cells. České Budějovice, 2018. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/52402